Tuesday, January 14, 2014
Data Analysis
Both our trees indicate that Rose and Jasmine, our two odorous plants, are older than our lily and alstroemeria (non-odorous), so we can assume that non-odorous plants evolved later from odorous plants. Our data mostly supports our hypothesis although our phylogeny tree could have multiple errors.
Conclusion Analysis
Our phylogeny trees, although not completely matching up, are very similar. Because we didnt know genus of all our plants (rose or alstroemeria) we couldn't be absolute when we were finding protein sequences, so we can't be sure our trees/ data accurately reflect the evolutionary order of our species, but it does seem to appear that odorous and non odorous plants branched off from multiple lineages which follows our hypothesis.
In both trees the non-odorous plants, Lily and Alstroemeria, evolved later from the odorous plants. This possibly suggests that at some point odorous plant lost their fragrance due to an environmental pressure to do so or perhaps lack thereof.
Our Morphology tree shows Alstroemeria evolving before the Aisan lily, but the protein sequence tree indicates the opposite. We are more inclined to trust the protein sequence more since it displays a hard science rather than an evaluative observation.
In both trees the non-odorous plants, Lily and Alstroemeria, evolved later from the odorous plants. This possibly suggests that at some point odorous plant lost their fragrance due to an environmental pressure to do so or perhaps lack thereof.
Our Morphology tree shows Alstroemeria evolving before the Aisan lily, but the protein sequence tree indicates the opposite. We are more inclined to trust the protein sequence more since it displays a hard science rather than an evaluative observation.
Thursday, January 9, 2014
source articles
Evolution of reproductive strategies in
the sexually deceptive orchid Ophrys sphegodes: how does flower-specific
variation of odor signals influence reproductive success?
This article
examines the reproductive stratagies of orchids in artracting bees to transport
pollen.
The hidden language of flowering
plants: floral odors as a key for
Wake Up and Smell the Roses: The Ecology
and Evolution of Floral Scent
A study of the history of floral odors of
plants in relation to their flower appearances
Evolution of reproductive strategies in
the sexually deceptive orchid Ophrys sphegodes: how does flower-specific
variation of odor signals influence reproductive success?
This article
examines the reproductive stratagies of orchids in artracting bees to transport
pollen.
The hidden language of flowering
plants: floral odors as a key for
understanding angiosperm evolution.
Wake Up and Smell the Roses: The Ecology
and Evolution of Floral Scent
A study of the history of floral odors of
plants in relation to their flower appearances
Tuesday, January 7, 2014
Pollen Morphology
Pollen Morphology Table
| Common Name | Scientific Name | Pollen size (μm) | Pollen Type | Number of Apertures | Surface Texture |
| Asian Lily | Lilium asiatica | Length 106 | monocolpate | 1 | clavate structures with reticulate patterns |
| width 42.6 | |||||
| Rose | Rosa sp. | length 29.5 | tricolpate (may be incorrect) | 3 | striate |
| width 14 | |||||
| Jasmine | Jasminum sp. | length 38.5 | monocolpate | 2 | Ruguate |
| Alstroemeria | Alstroemeria aurea | length 54.6 | monolete | 1 | striate |
| width 23.6 |
We organized this phylogeny tree based on the number of morphological similarities between each species. The tree begins with Jasmine who's pollen doesn't share any similarities with the other plants. Next, our rose had 1 similarity, it has the same striated surface texture as Alstoremeria. We put the Asian lily which is a monocolpate and has one aperture, like Alstroemeria next. Lastly we were left with Alstroemeria, which shared three similarities with the other plants.
Thursday, December 19, 2013
Procedure
1. We decided which flowers we wanted to study the pollen of.
2. The flowers chosen were collected and then dried in a dehydrator.
3. After the flowers finished drying, we harvested each flower's pollen.
4. Once harvested, we put the portion of the pollen under a microscope and wrote down their features and measurements at 40x magnification.
5. When we were finished viewing the pollen under the microscope we placed the remainder of the sample on a stub for SEM viewing.
6. We then prepared the stub for the SEM by carefully carried out the steps instructed to us.
7.Upon completion of the stub preparation process, we then put the stub in the SEM cup and made it flush with the rim.
8. The cup was then ready to be inserted into the SEM(the FEI Phenom pro).
9. once prepared, we inserted the cup and stub into the SEM and started the scan.
10. We then took pictures of each pollen sample and measured them at 2000x magnification. We also took pictures at a closer magnification for extreme close up detail.
2. The flowers chosen were collected and then dried in a dehydrator.
3. After the flowers finished drying, we harvested each flower's pollen.
4. Once harvested, we put the portion of the pollen under a microscope and wrote down their features and measurements at 40x magnification.
5. When we were finished viewing the pollen under the microscope we placed the remainder of the sample on a stub for SEM viewing.
6. We then prepared the stub for the SEM by carefully carried out the steps instructed to us.
7.Upon completion of the stub preparation process, we then put the stub in the SEM cup and made it flush with the rim.
8. The cup was then ready to be inserted into the SEM(the FEI Phenom pro).
9. once prepared, we inserted the cup and stub into the SEM and started the scan.
10. We then took pictures of each pollen sample and measured them at 2000x magnification. We also took pictures at a closer magnification for extreme close up detail.
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