Procedure

1. We decided which flowers we wanted to study the pollen of.
2. The flowers chosen were collected and then dried in a dehydrator.
3. After the flowers finished drying, we harvested each flower's pollen.
4. Once harvested, we put the portion of the pollen under a microscope and wrote down their features and measurements at 40x magnification.
5. When we were finished viewing the pollen under the microscope we placed the remainder of the sample on a stub for SEM viewing.
6. We then prepared the stub for the SEM by carefully carried out the steps instructed to us.
7.Upon completion of the stub preparation process, we then put the stub in the SEM cup and made it flush with the rim.
8. The cup was then ready to be inserted into the SEM(the FEI Phenom pro).
9. once prepared, we inserted the cup and stub into the SEM and started the scan.
10. We then took pictures of each pollen sample and measured them at 2000x magnification. We also took pictures at a closer magnification for extreme close up detail.

Odorous Rose pollen taken on SEM at 2000x. Taken by Grant Lamberton, Sam Wanke, and Jane Stockbridge.

Odorous Rose pollen taken on SEM at 2000x with measurements. Length: 29.5 micrometers. Width: 14 micrometers. Taken by Grant Lamberton, Sam Wanke, and Jane Stockbridge.

Odorous Rose pollen taken on SEM at 9900x. Taken by Grant Lamberton, Sam Wanke, and Jane Stockbridge.

Asian Lilly pollen taken on SEM at 380x. Taken by Jane Stockbridge, Grant Lamberton, and Sam Wanke.

Asian Lilly pollen taken on SEM at 2000x. Taken by Jane Stockbridge, Grant Lamberton, and Sam Wanke.

Asian Lilly pollen taken on SEM at 2000x with measurements. Length: 106 micrometers. Width: 42.6 micromters. Taken by Jane Stockbridge, Grant Lamberton, and Sam Wanke.

Asian Lilly pollen taken on SEM at 4750x. Taken by Jane Stockbridge, Grant Lamberton, and Sam Wanke.

Asian Lilly pollen taken on SEM at 10000x. Taken by Jane Stockbridge, Grant Lamberton, and Sam Wanke.

Jasmine pollen on SEM at 2000x. Taken by Sam Wanke, Jane Stockbridge, and Grant Lamberton.

Jasmine pollen taken on SEM at 2000x with measurement of 38.5 micrometer. Taken by Sam Wanke, Jane Stockbridge, and Grant Lamberton

Jasmine pollen taken on SEM at 10000x. Taken by Sam Wanke, Jane Stockbridge, and Grant Lamberton.

Jasmine pollen taken by SEM at 10000x. Taken by Sam Wanke, Jane Stockbridge, and Grant Lamberton.

Team Hypothesis

We hypothesize that not all flowers come from the same ancestor, but that all all odorous ones do.

Alstroemeria pollen on SEM at 2000x. Taken by Grant Lamberton, Sam Wanke, and Jane Stockbridge

Alstroemeria pollen on SEM at 3550x. Taken by Grant Lamberton, Sam Wanke, and Jane Stockbridge

Alstroemeria pollen on SEM at 8550x. Taken by Grant Lamberton, Sam Wanke, and Jane Stockbridge

Measurements of Alstroemeria pollen on SEM at 2000x. Length: 54.6 micrometers. Width: 23.6 micrometers. Taken by Grant Lamberton, Sam Wanke, and Jane Stockbridge

Team agreement

Our team agrees to share work evenly and help our group to research our question and post on our blog.

Grant: SEM operator
Sam: SEM photographer
Jane: Data collector and recorder

Addendum
 Dec. 10th
     Grant and Sam researched and found articles about research on odorous plants.

 Dec. 12th
     Grant and Jane took photos at 35x of the pollen.

 Dec. 16th 
     We all began working on the how microscopes work form.

 Dec. 17th
     Worked with the SEM today, getting 3D photos of all our pollens' surfaces at 2000x (and higher.)           Jane also uploaded more of the photos taken from Dec. 12

 Dec. 19th
     Grant and Jane continued completing blog by posting more pictures and data.

 Jan. 6th
     We began filling out a pollen data spreadsheet and formulating a phylogenic tree bases on physical characteristics of our pollen. We all worked collectively on the blog making sure we had details all covered.

 Jan. 7th
     Finished the pollen spreadsheet and used UniProt to develop a phylogenic tree of our pollen based     on amino acid chains.

 Jan. 9th
     worked through our blog checklist updating entries and making sure we had all the requirements.

Alstroemeria pollen taken on a Leica at 35x by Grant Lamberton and Jane Stockbridge

Alstroemeria pollen taken on Leica at 35x by Grant Lamberton and Jane Stockbridge

Jasmine pollen taken on Leica at 35x by Grant Lamberton and Jane Stockbridge

Asian Lilly anther, taken by a Leica Microscope. Picture by Jane Stockbridge and Grant Lamberton

Closer view of Asian Lilly anther, taken by a Leica Microscope. Picture by Jane Stockbridge and Grant Lamberton

Asian Lilly pollen taken on a Leica at 35x by Jane Stockbridge and Grant Lamberton