Procedure

1. We decided which flowers we wanted to study the pollen of.
2. The flowers chosen were collected and then dried in a dehydrator.
3. After the flowers finished drying, we harvested each flower's pollen.
4. Once harvested, we put the portion of the pollen under a microscope and wrote down their features and measurements at 40x magnification.
5. When we were finished viewing the pollen under the microscope we placed the remainder of the sample on a stub for SEM viewing.
6. We then prepared the stub for the SEM by carefully carried out the steps instructed to us.
7.Upon completion of the stub preparation process, we then put the stub in the SEM cup and made it flush with the rim.
8. The cup was then ready to be inserted into the SEM(the FEI Phenom pro).
9. once prepared, we inserted the cup and stub into the SEM and started the scan.
10. We then took pictures of each pollen sample and measured them at 2000x magnification. We also took pictures at a closer magnification for extreme close up detail.